Extended depth-of-field imaging and ranging in a snapshot

Traditional approaches to imaging require that an increase in depth of field is associated with a reduction in numerical aperture, and hence with a reduction in resolution and optical throughput. In their seminal work, Dowski and Cathey reported how the asymmetric point-spread function generated by a cubic-phase aberration encodes the detected image such that digital recovery can yield images with an extended depth of field without sacrificing resolution [Appl. Opt. 34, 1859 (1995)]. Unfortunately recovered images are generally visibly degraded by artifacts arising from subtle variations in point-spread functions with defocus. We report a technique that involves determination of the spatially variant translation of image components that accompanies defocus to enable determination of spatially variant defocus. This in turn enables recovery of artifact-free, extended depth-of-field images together with a two-dimensional defocus and range map of the imaged scene. We demonstrate the technique for high-quality macroscopic and microscopic imaging of scenes presenting an extended defocus of up to two waves, and for generation of defocus maps with an uncertainty of 0.036 waves

Video-rate computational super-resolution and integral imaging at longwave-infrared wavelengths

We report the first computational super-resolved, multi-camera integral imaging at long-wave infrared (LWIR) wavelengths. A synchronized array of FLIR Lepton cameras was assembled, and computational super-resolution and integral-imaging reconstruction employed to generate video with light-field imaging capabilities, such as 3D imaging and recognition of partially obscured objects, while also providing a four-fold increase in effective pixel count. This approach to high-resolution imaging enables a fundamental reduction in the track length and volume of an imaging system, while also enabling use of low-cost lens materials.Comment: Supplementary multimedia material in http://dx.doi.org/10.6084/m9.figshare.530302

Fourier Ptychography with Scheimpflug Optics for Multi-Aperture Applications

We present a new optical configuration using the Scheimpflug principle for Fourier ptychography microscopy. This configuration minimizes the aberrations present in the off-axis lenses of a multi-aperture Fourier ptychography setup. A 3D printed setup was used to demonstrate the experimental implementation

Super-resolution imaging using a camera array

The angular resolution of many commercial imaging systems is limited, not by diffraction or optical aberrations, but by pixilation effects. Multiaperture imaging has previously demonstrated the potential for super-resolution (SR) imaging using a lenslet array and single detector array. We describe the practical demonstration of SR imaging using an array of 25 independent commercial-off-the-shelf cameras. This technique demonstrates the potential for increasing the angular resolution toward the diffraction limit, but without the limit on angular resolution imposed by the use of a single detector array

Compact multi-aperture imaging with high-angular-resolution

Previous reports have demonstrated that it is possible to emulate the imaging function of a single conventional lens with an NxN array of identical lenslets to provide an N-fold reduction in imaging-system track length. This approach limits the application to low-resolution imaging. We highlight how using an array of dissimilar lenslets, with an array width that can be much wider than the detector array, high-resolution super-resolved imaging is possible. We illustrate this approach with a ray-traced design and optimization of a long-wave infrared system employing a 3x3 array of free-form lenslets to provide a four-fold reduction in track length compared to a baseline system. Simulations of image recovery show that recovered image quality is comparable to that of the baseline system

Computational localization microscopy with extended axial range

A new single-aperture 3D particle-localization and tracking technique is presented that demonstrates an increase in depth range by more than an order of magnitude without compromising optical resolution and throughput. We exploit the extended depth range and depth-dependent translation of an Airy-beam PSF for 3D localization over an extended volume in a single snapshot. The technique is applicable to all bright-field and fluorescence modalities for particle localization and tracking, ranging from super-resolution microscopy through to the tracking of fluorescent beads and endogenous particles within cells. We demonstrate and validate its application to real-time 3D velocity imaging of fluid flow in capillaries using fluorescent tracer beads. An axial localization precision of 50 nm was obtained over a depth range of 120μm using a 0.4NA, 20× microscope objective. We believe this to be the highest ratio of axial range-to-precision reported to date

Miniature Fourier Ptychography Microscope using Raspberry Pi Camera and Hardware

We report a Fourier ptychography setup using a raspberry pi camera sensor and its lens in reversed configuration. In this work data acquisition was performed by means of a raspberry pi board which eliminates the requirement of a computer for data acquisition thus allowing a miniaturized system for remote data acquisition costing around £100

Multispectral oximetry of murine tendon microvasculature with inflammation

We report a novel multispectral imaging technique for localised measurement of vascular oxygen saturation (SO2) in vivo. Annular back-illumination is generated using a Schwarzchild-design reflective objective. Analysis of multispectral data is performed using a calibration-free oximetry algorithm. This technique is applied to oximetry in mice to measure SO2 in microvasculature supplying inflamed tendon tissue in the hind leg. Average SO2 for controls was 94.8 ± 7.0 % (N = 6), and 84.0 ± 13.5 % for mice with inflamed tendon tissue (N = 6). We believe this to be the first localised measurement of hypoxia in tendon microvasculature due to inflammation. Quantification of localised SO2 is important for the study of inflammatory diseases such as rheumatoid arthritis, where hypoxia is thought to play a role in pathogenesis